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hydroxyethyl 1 piperazineethanesulfonic acid hepes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hydroxyethyl 1 piperazineethanesulfonic acid hepes
    Hydroxyethyl 1 Piperazineethanesulfonic Acid Hepes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepes+buffer+1/HEPES%2C+0%2E5M+buffer+soln%2E%2C+pH+7%2E5/pm42097025-210-30-39
    Average 96 stars, based on 1 article reviews
    hydroxyethyl 1 piperazineethanesulfonic acid hepes - by Bioz Stars, 2026-10
    96/100 stars

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    Cell Culture:

    Article Title: High-throughput drug screening in advanced pre-clinical 3D melanoma models identifies potential first-line therapies for NRAS-mutated melanoma
    Article Snippet: Patient-derived human melanoma cell lines M160915 and M161022 (Prof. Mitchell Levesque, University Hospital Zurich , Switzerland) were cultured in RPMI 1640 (Gibco Thermo Fisher Scientific, USA), supplemented with 10% FCS, 1mM Sodium Pyruvate (Gibco Thermo Fisher Scientific, USA), 4mM L-Glutamine (Gibco Thermo Fisher Scientific, USA), and 0.1 mg/mL Normocin. .. NHDF (normal human dermal fibroblasts) (Promocell, C-12300), MRC-5 (human lung fibroblasts) (ATCC, CCL-171), and LX-2 cells (human hepatic stellate cells) (Merk, SCC064) were cultured in DMEM enriched with GlutaMAX (Gibco Thermo Fisher Scientific, USA), supplemented with 10% FCS, 2.5% HEPES buffer 1 M (Gibco Thermo Fisher Scientific, USA), and 0.1 mg/mL Normocin. .. HMEC-1 (human endothelial cells) (ATCC, CRL-3243) were cultured in MCDB131 (Gibco Thermo Fisher Scientific, USA), supplemented with 10% FCS, 1 μg/mL Hydrocortisone (Sigma-Aldrich, USA), 10mM L-Glutamine, 0.1 mg/mL Normocin, and 10 ng/mL recombinant human EGF (PeproTech, USA).

    Article Title: Identification of ACBP as a potential target in ciliopathic obesity through multi-omics network analysis
    Article Snippet: Each slide was examined using a Zeiss Axiophot microscope. .. HepG2 cells (HB-806, ATCC) obtained from human hepatocellular carcinoma were cultured at 37 °C under 5% CO 2 in Dulbecco’s Modified Eagle Medium (DMEM) (41966029, Gibco) containing D-glucose 25 mM, sodium pyruvate 1 mM, L-glutamine 4 mM, and supplemented with 10% heat-inactivated fetal bovine (10500064, Gibco), HEPES buffer 1 M (15630056, Gibco), and 10000 units/mL penicillin G sodium and 100000 μg/mL streptomycin sulfate (15140122, Gibco). .. Cells were seeded in 6–12-well plates and grown for 36 h before treatment with 50 nM BafA1 (S-S1413, Selleckchem), EBSS medium (24010043, Gibco) or anti-ACBP (or respective isotype control) in vitro (1–10 μg/mL; ab232760, Abcam).

    Modification:

    Article Title: Identification of ACBP as a potential target in ciliopathic obesity through multi-omics network analysis
    Article Snippet: Each slide was examined using a Zeiss Axiophot microscope. .. HepG2 cells (HB-806, ATCC) obtained from human hepatocellular carcinoma were cultured at 37 °C under 5% CO 2 in Dulbecco’s Modified Eagle Medium (DMEM) (41966029, Gibco) containing D-glucose 25 mM, sodium pyruvate 1 mM, L-glutamine 4 mM, and supplemented with 10% heat-inactivated fetal bovine (10500064, Gibco), HEPES buffer 1 M (15630056, Gibco), and 10000 units/mL penicillin G sodium and 100000 μg/mL streptomycin sulfate (15140122, Gibco). .. Cells were seeded in 6–12-well plates and grown for 36 h before treatment with 50 nM BafA1 (S-S1413, Selleckchem), EBSS medium (24010043, Gibco) or anti-ACBP (or respective isotype control) in vitro (1–10 μg/mL; ab232760, Abcam).



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